HPLC Column, RP C4 Butyl, Wide Pore, 3um, 2.1mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4 Butyl™ Wide Pore is a reversed-phase HPLC stationary phase specifically designed for biomolecule separations. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, this 300 Å wide pore chemistry provides improved accessibility for larger molecules compared to standard 100 Å pore columns. The result is enhanced mass transfer and chromatographic performance for proteins, peptides, enzymes, and other large biomolecules that may be restricted by smaller pore structures.
68304-05P-2-3M is an HPLC column packed with 3 µm RP C4 Butyl™ Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 50 mm format. This popular narrow-bore configuration is optimized for rapid bioanalytical separations with reduced solvent consumption. The 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns, while the short 50 mm length supports high-throughput workflows. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, HPLC systems must be capable of precise and reproducible low-flow operation.
This column is ideal for proteins, peptides, enzymes, and other large hydrophobic or neutral compounds analyzed by reversed-phase chromatography. The narrow-bore format is also well suited for LC-MS applications where sensitivity, reduced solvent use, and rapid analysis are important.
Key Benefits
RP C4 Butyl™ Wide Pore stationary phase
Fully double end capped
Traditional butyl (C4) bonded phase
Ultra-high purity silica support
300 Å wide pore design
Optimized for proteins and peptides
3 µm particle technology
Greater efficiency than 5 µm columns
Fast analysis times
Excellent for bioanalytical applications
Typical Applications
Protein analysis
Peptide analysis
Enzyme characterization
Bioanalytical methods
LC-MS methods
Biopharmaceutical analysis
Protein purity testing
Biomolecule separations
Method development
Reversed-phase HPLC
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4 Butyl™ Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 2.1 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 0.2–0.3 mL/min
Brand: Cogent
Important Notes
Designed for proteins, peptides, enzymes, and other large biomolecules
300 Å wide pores improve access to the bonded phase for larger molecules
Suitable for conventional HPLC and LC-MS systems
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Generates higher backpressure than larger particle columns
The 2.1 mm ID format reduces solvent consumption and is frequently used with LC-MS systems
Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min
Detector flow cells should be optimized for low-volume, narrow-bore chromatography
Ideal for rapid bioanalytical and biopharmaceutical workflows